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In this article we will discuss about the process of androgenesis, explained with the Success of anther culture is highly dependent on the genotype of the plant. The success of androgenesis dependent on the variety used, the growth . Razdan M.K, An introduction to plant tissue culture, ().Oxford. In vitro androgenesis is an important component of plant biotechnology when the pollen .. In: Korea-China Plant Tissue Culture Symposium, Academia Sinica.

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Process of Androgenesis (With Diagram) | Biotechnology

In this article we will discuss about the process of androgenesis, explained with the help of suitable diagrams. Microspores undergoes division in unorganised fashion to give rise to callus and by embryogenic or organogenic induction the haploid plantlets may be obtained.

There are four cilture pathways to form the multicellular condition of pollen from the unicellular pollen Fig.

The uninucleate microspores divide unequally forming vegetative and generative cell. The sporophyte arises through divisions of vegetative cell, the generative cell gets degenerated e. The uninucleate microspore undergoes a normal unequal division but the pollen embryos are formed from the generative cell alone. The vegetative cell does not divide, e.

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Production of Haploid Plants (With Diagram)

The division of microspore is asymmetrical as in pathway II, but both the cells take part in embryo formation and sporophyte development e.

Anthers with microspores ranging from tetrad to the bi-nucleate stage are responsive to culture. As soon as the starch deposition starts within the microspore there is androgejesis further development towards sporophyte.

The anther should llant collected from the flower buds of adult healthy plant and it is very important to use the healthy explant. The andgogenesis in response of anthers from plants grown under different environmental conditions may be due to the differences in endogenous level of growth regulators.

Flowers from relatively younger plants, flowering at the beginning of flower-season are more responsive. It has been observed that various species and cultivars exhibit different growth responses in culture. The temperature shock helps in dissolution of microtubules and causes abnormal division of microspores.

Chemicals like 2-chloroethyl phosphonic acid have a pronounced effect in increasing the haploid production in various species. It helps to develop the multinucleate condition with fewer starch grains.

Composition of medium is one of the most important factors determining the success of anther culture.

Process of Androgenesis (With Diagram) | Biotechnology

Minerals and growth regulators play important roles on embryogenesis but it totally depends on the endogenous level of hormones. After formation of macroscopic structures, these can be transferred to a regeneration medium and kept at 14 hr.

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This variation may be due to endomitosis or fusion of various nuclei during the developmental stages of anthers at the time of excision and culture. For obtaining homozygous diploid lines, the plants derived through anther culture must be analysed for their ploidy level and then the following methods can be applied: As colchicine is a spindle inhibitor it is used to induce chromosome duplication in various ways: Haploids are in general unstable in culture and have a tendency to undergo endomitosis to form diploid cells.

This property can be exploited in some cases to obtain the homozygous plant. This is a question and answer forum for students, teachers and general visitors for exchanging articles, answers and notes.

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